1. The _________ test involves using rabbit plasma as a reagent and its results are determined by observation of thickening of the medium.

A. Catalase

B. Oxidase

C. Coagulase

D. Peroxidase

Answer: Coagulase

2. Gram-positive cocci in chains would be called staphylococci.

True

False

Answer: True

3. Which of the following bacterial species would be most likely to grow in bile esculin medium and hydrolyze the esculin?

A. Enterococcus faecalis

B. Streptococcus pneumoniae

C. Staphylococcus aureus

D. Staphylococcus epidermidis

Answer: Enterococcus faecalis

4. Identification of a bacterial species cannot be made on microscopic morphology alone because __________.

A. Bacterial shapes are usually variable and are not a reliable characteristic for identification

B. Many organisms have a similar microscopic morphology

C. Staining reactions are variable and not a reliable characteristic

D. Staining and microscopic observation is too time-consuming for routine use

Answer: Many organisms have a similar microscopic morphology

5. The absence of hemolysis on a blood agar plate is referred to as _______ hemolysis

A. Alpha

B. Beta

C. Gamma

D. Delta

Answer: Gamma

6. Which of the following is a characteristic of members of the resident microbiota?

A. Resident microbiota can compete with and limit growth of harmful bacteria.

B. Resident microbiota are a major source of nutrients for our body.

C. Resident microbiota typically cause serious infections.

D. Resident microbiota often initiate allergic reactions.

Answer: Resident microbiota can compete with and limit growth of harmful bacteria.

7. When a large zone of no growth is found around an antibiotic disc being used for identification purposed, this generally means that the microbe is ______ to the antibiotic

A. Resistant

B. Pathogenic

C. Sensitive

D. Neutral

Answer: Sensitive

8. All of the following infections are frequently cause by Staphylococcus and Streptococcus species EXCEPT?

A. Endocarditis

B. Abscess

C. Diarrhea

D. Pneumonia

Answer: Diarrhea

9. Which test uses a medium that is both selective and differential for members of the genus Staphylococcus?

A. MacConkey agar

B. Mannitol Salt agar

C. Coagulase test

D. Bile esculin hydrolysis

Answer: Mannitol Salt agar

10. All of the following are methods that can be used to isolate bacteria from a mixed culture EXCEPT?

A. Streak plating

B. Loop dilution pour plating

C. Gram staining

D. Serial dilution followed by spread plating

Answer: Gram staining

11. How do you determine the species of an unknown bacterium?

A. You should make a careful investigation of the morphology of the bacterial cells using a microscope to make a firm identification.

B. You need to quantify the number of bacteria in the same to determine the species.

C. Identification can only be made based on the patient’s signs and symptoms.

D. You use standard tests and pre-existing knowledge to classify the bacterium.

Answer: You use standard tests and pre-existing knowledge to classify the bacterium.

12. All of the following body sites normally have large numbers of resident microbiota EXCEPT?

A. Nose

B. Large intestine

C. Skin

D. Blood

Answer: Blood

13. The field of study in which medicine and microbiology intersect is known as medical microbiology

True

False

Answer: True

14. A microorganisms that is most often harmless but may occasionally cause infection and disease if defenses are compromised is referred to as an opportunistic pathogen.

True

False

Answer: True

15. Which test utilizes hydrogen peroxide and the observation of gas bubble formation when attempting to identify an unknown bacterium?

A. Bile esculin hydrolysis

B. Coagulase test

C. Oxidase test

D. Catalase test

Answer: Catalase test

16. A bacterial isolate grown on a mannitol salt agar plate changes the pink agar to yellow and produces bright yellow colonies. This indicates that the isolate is _______ for mannitol fermentation.

Negative

Positive

Answer: Positive

17. Impetigo, a type of inflammatory skin infection, is caused by__________

A. Enterococcus faecalis

B. Streptococcus pneumoniae

C. Staphylococcus aureus

E. coli

Answer: Staphylococcus aureus

18. The complete clearing or hemolysis that a bacterial isolate may produce on blood agar is called alpha hemolysis

True

False

Answer: True

19. The Gram stain is an example of a test that can be used to determine the ______ characteristics of a bacterium?

A. Physiological

B. Genetic

C. Morphological

D. Culturing

Answer: Morphological

20. Before attempting to identify an unknown bacterial species, the organism must be isolated in a pure culture.

True

False

Answer: True

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